mouse monoclonal anti cdk6  (Cell Signaling Technology Inc)

Journal: Cell reports

Article Title: Hepatic Snai1 and Snai2 promote liver regeneration and suppress liver fibrosis in mice

doi: 10.1016/j.celrep.2024.113875

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Article Snippet: Mouse monoclonal anti-CDK6 , Cell Signaling Technology , Cat#3136; RRID:AB_2229289.

Techniques: Plasmid Preparation, Virus, In Situ, Real-time Polymerase Chain Reaction, Recombinant, Luciferase, Software

Journal: Cell Reports Medicine

Article Title: Co-targeting RANK pathway treats and prevents acquired resistance to CDK4/6 inhibitors in luminal breast cancer

doi: 10.1016/j.xcrm.2023.101120

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Article Snippet: Mouse monoclonal anti CDK6 (DCS83) , Cell Signaling , Cat#3136; RRID:AB_2229289.

Techniques: Recombinant, Control, shRNA, Blocking Assay, Alamar Blue Assay, Flow Cytometry, Isolation, cDNA Synthesis, Bicinchoninic Acid Protein Assay, TUNEL Assay, Enzyme-linked Immunosorbent Assay, Gene Expression, Western Blot, Software

Journal: Animals : an Open Access Journal from MDPI

Article Title: SV40T/E6E7-Induced Proliferation Is Involved in the Activity of E2F3 in Bovine Mammary Epithelial Cells

doi: 10.3390/ani12141790

Figure Lengend Snippet: Involvement of E2F signaling pathway in the proliferation of the immortalized BMECs. ( A ) Western blotting analysis of CDK4, CDK6, cyclin D1, cyclin D3, and CDK2 in primary and immortalized BMECs. ( B ) Western blotting analysis of E2F1, E2F2, and E2F3 in primary and immortalized BMECs. Primary BMECs or BMECs immortalized by E6E7 and SV40T were collected at early (E) and late (L) passages of culture. Data are based on triplicate experiments.

Article Snippet: The following primary antibodies were obtained from Cell Signaling Technology (CST, Shanghai, China): rabbit anti-β-actin monoclonal antibody (diluted 1:1000; #4970), rabbit anti-GAPDH monoclonal antibody (1:1000; #2118), rabbit anti-cyclin D1 monoclonal antibody (1:1000; #2978), rabbit anti-CDK2 monoclonal antibody (1:1000; #2546), mouse anti-CDK6 monoclonal antibody (1:1000; #3136), rabbit anti-cyclin D3 monoclonal antibody (1:1000; #2936), rabbit anti-CDK4 monoclonal antibody (1:1000; #12790), and rabbit anti-p27 Kip1 monoclonal antibody (1:1000; #3686).

Techniques: Western Blot

Journal: Frontiers in Cell and Developmental Biology

Article Title: Recreating the Bone Marrow Microenvironment to Model Leukemic Stem Cell Quiescence

doi: 10.3389/fcell.2021.662868

Figure Lengend Snippet: Hypoxia and co-culture with bone marrow mesenchymal stromal cells (BMSC) + TGFβ-1 increases the expression of markers of leukemia cell quiescence. KG1a cells were cultured alone or with alginate-encapsulated BMSC and TGFβ-1 (10 ng/ml) over a 12-day period in normoxia or hypoxia (1% pO 2 ). (A) Expression of CD150 determined in the live fraction by flow cytometry. MFI, mean fluorescent intensity. * p < 0.05 (Student’s t -test). (B–E) Results of quantitative RT-PCRs on RNA isolated on days 8 and 12. * p < 0.05 (one-way ANOVA). (F) Gene expression analysis of p21 expression conducted on the GSE17054 transcriptomic dataset. The graph represents the changes in gene expression between hematopoietic stem cells and leukemic stem cells. * p < 0.05 (Welch’s t -test). (G) Western blot images for the expression of p21, CDK4, and CDK6 conducted on samples isolated at day 12 of culture. SN, single culture at normoxia; CH, co-culture under hypoxia.

Article Snippet: After blocking, the blots were incubated with rabbit monoclonal antibodies against CDK4 [Cell Signaling Technology (CST)] and p21 (Santa Cruz) and with mouse monoclonal antibodies against CDK6 (CST) and actin (Sigma-Aldrich).

Techniques: Co-Culture Assay, Expressing, Cell Culture, Flow Cytometry, Isolation, Gene Expression, Western Blot